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| Registros recuperados: 12 | |
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| NGUYEN, Thu-Thuy; MOTSIRI, Mono Sophie; TAIOE, Moeti Oriel; MTSHALI, Moses Sibusiso; GOTO, Yasuyuki; KAWAZU, Shin-Ichiro; Molifi THEKISOE, Oriel Matlhahane; INOUE, Noboru; 河津, 信一郎; 井上, 昇. |
| A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant... |
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Palavras-chave: Animal African trypanosomosis; ELISA; Immunochromatographic test; Serodiagnosis; South Africa. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4034 |
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| MOSSAAD, Ehab; ASADA, Masahito; NAKATANI, Daichi; INOUE, Noboru; YOKOYAMA1), Naoaki; KANEKO, Osamu; KAWAZU, Shin-ichiro; 井上, 昇; 横山, 直明; 河津, 信一郎. |
| Bovine babesiosis is a livestock disease known to cause economic losses in endemic areas. The apicomplexan parasite Babesia bovis is able to invade and destroy the host’s erythrocytes leading to the serious pathologies of the disease, such as anemia and hemoglobinuria. Understanding the egress mechanisms of this parasite is therefore a key step to develop new therapeutic strategies. In this study, the possible involvement of Ca2+ in the egress of B. bovis merozoites from infected erythrocytes was investigated. Egress was artificially induced in vitro using calcium ionophore A23187 and thapsigargin to increase Ca2+ concentration in the cytosol of the parasite cells. The increased intracellular Ca2+ concentration following these treatments was confirmed... |
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Palavras-chave: Babesia bovis; Calcium ionophore; Calcium signalling; Egress. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4071 |
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| MASATANI, Tatsunori; ASADA, Masahito; ICHIKAWA-SEKI, Madoka; USUI, Miho; TERKAWI, Mohamad A.; HAYASHI, Kei; KAWAZU, Shin-ichiro; XUAN, Xuenan; 河津, 信一郎; 玄, 学南. |
| Peroxiredoxins (Prxs) are a family of antioxidant enzymes. Here, we cloned a 2-Cys Prx, BgTPx-1, from the canine Babesia parasite B. gibsoni. Sequence identity between BgTPx-1 and 2-Cys Prx of B. bovis was 81% at the amino acid level. Enzyme activity assay by using recombinant BgTPx-1 (rBgTPx-1) indicated that BgTPx-1 has antioxidant activity. Antiserum from a mouse immunized with rBgTPx-1 reacted with parasite lysates and detect a protein with a monomeric size of 22 kDa and also a 44 kDa protein, which might be an inefficiently reduced dimer. BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni. |
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Palavras-chave: Antioxidant activity; Babesia gibsoni; Canine; Peroxiredoxin. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3925 |
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| Hakimi, Hassan; Nguyen, Thu-Thuy; Suganuma, Keisuke; Masuda-Suganuma, Hirono; Ma. M. Angeles, Jose; Inoue, Noboru; Kawazu, Shin-ichiro; 井上, 昇; 河津, 信一郎. |
| Prompt and accurate diagnosis of malarial patients is a crucial factor in controlling the morbidity and mortality of the disease. Effective treatment decisions require a correct diagnosis among mixed-species malarial patients. Differential diagnosis is particularly important in cases of Plasmodium vivax, a species that shares endemicity with P. falciparum in most endemic areas. Moreover, it is difficult to identify P. knowlesi on the basis of morphology alone, and rapid diagnostic tests are still not available for this malaria species. Therefore, the development of diagnostic tests applicable to the field is urgently needed. 1-Cys peroxiredoxin (1-Cys-Prx) in P. falciparum is abundantly expressed in the mature asexual stages, making it a promising... |
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Palavras-chave: Malaria diagnosis; Monoclonal antibody; Peroxiredoxin; Plasmodium knowlesi; P. vivax. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3859 |
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| SUGANUMA, Keisuke; ALLAMANDA, Puttik; HAKIMI, Hassan; ZHOU, Mo; ANGELES, Jose Ma.; KAWAZU, Shin-ichiro; INOUE, Noboru; 河津, 信一郎; 井上, 昇. |
| Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well... |
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Palavras-chave: Animal African trypanosomosis; Drug screening; Luciferase assay; Trypanosoma congolense. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3984 |
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| YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; YBAÑEZ, Rochelle Haidee Daclan; RATILLA, Jowarren Catingan; PEREZ, Zandro Obligado; GABOTERO, Shirleny Reyes; HAKIMI, Hassan; KAWAZU, Shin-ichiro; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 河津, 信一郎; 松本, 高太郎; 横山, 直明; 猪熊, 壽. |
| Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale... |
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Palavras-chave: Anaplasma marginale; Cattle; PCR; Philippines; Rhipicephalus (Boophilus) microplus. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3866 |
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| Terkawi, M. A.; Aboge, G.; Jia, H.; Goo, Y-K.; Ooka, H.; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawazu, Shin-ichiro; Fujisaki, K.; Xuan, Xuenan; 山岸, 潤也; 西川, 義文; 河津, 信一郎; 玄, 学南. |
| A novel gene encoding 27-kDa protein was identified by the screening of Babesia gibsoni cDNA library with acutely infected dog serum. The BgP27 is a single copy gene with a predicted open reading frame of 762 bp and 254 amino acids. The phylogenic analysis of the deduced amino acid of BgP27 demonstrated considerable identities with members of Plasmodium berghei circumsporozoite protein family that ranged between 18.4% and 22.8%. The BgP27 was expressed as a glutathione S-transferase fusion protein in Escherichia coli. The serum raised in mice against the recombinant protein specifically reacted with a 27-kDa protein in the extracts of B. gibsoni parasites. Confocal laser scanning microscopic observation showed high fluorescent reactivity with both... |
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Palavras-chave: Babesia gibsoni; Enzyme-linked immunosorbent assay; Deiagnostic performance. |
| Ano: 2008 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2232 |
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| KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明. |
| Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3). |
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Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585 |
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| 河津, 信一郎; KAWAZU, SHIN-ICHIRO. |
| チオレドキシンペルオキシダーゼ2-Cys型Prx遺伝子を欠損するローデント（ネズミ）マラリア原虫について、これまでに観察された表現型発現のメカニズムを調べた。（１）哺乳類赤血球内でのガメートサイト（生殖母体）の分化誘導・形成阻害について：赤内期原虫タンパク質の二次元電気泳動解析から、この表現型にタンパク質リン酸化が関与することが示唆された。また（２）スポロゾイト（感染型原虫）の哺乳類宿主への感染性の低下について：マウス感染モデルを用いたRT-PCR解析から、この表現型が、スポロゾイトの障害ではなく、肝内型原虫の発育阻害に起因することが示唆された。 Phenotypes observed in the rodent malaria parasite Plasmodium berghei carrying a targeted knockout of thioredoxin peroxidase, 2-Cys peroxiredoxin gene were investigated and have the following results: (1) Defect in the gametocyte (sexual stage for transition to mosquitoes) development: 2D-PAGE analysis of the blood-stage parasite proteins suggested that the defect is attributed to the protein phosphorylation; (2) Extension in the prepatent time in the sporozoite (infective stage to... |
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Palavras-chave: 感染症; マラリア; ストレス; レドックス; 遺伝子改変原虫. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3031 |
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| Registros recuperados: 12 | |
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